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Image Search Results
Journal: Cell metabolism
Article Title: Elevated PGC-1α activity sustains mitochondrial biogenesis and muscle function without extending survival in a mouse model of inherited ALS.
doi: 10.1016/j.cmet.2012.03.019
Figure Lengend Snippet: Figure 4. Elevating PGC-1a Expression in Skeletal Muscle of SOD1G37R Mutant Mice Does Not Alter ALS Disease Course or Pathogenesis (A) Plot of ages (in days) at which disease onset (as determined by the weight peak; at onset, animals do not display any obvious motor phenotype), symptomatic stage (as determined by 10% weight loss from onset, a stage characterized by clear gait abnormalities and tremor) and end-stage (as determined by hindlimb paralysis and inability to right itself) were reached for SOD1G37R (red) and SOD1G37R/MCK-PGC-1a (blue) animals. (B–D) Quantification of innervation at the neuromuscular junction of the gastrocnemius muscle (B), total number of a-motor axons in the lumbar L5 motor root (C), and quantification at disease end-stage of the average number of large cholinergic ventral horn motor neurons per section of lumbar spinal cord from SOD1G37R and SOD1G37R/MCK-PGC-1a animals (D). Data are presented as mean ± SEM. See also Figure S4 for representative sections of the spinal cords used for quantification. (E) Representative micrographs of lumbar spinal cord sections from SOD1G37R and SOD1G37R/MCK-PGC-1a animals at disease end-stage processed for immuno- fluorescence using antibodies detecting activated astrocytes (GFAP) or microglia (IbaI). Dashed outlines correspond to the boundary between gray and white matter.
Article Snippet: Immunoblotting PGC-1a was immunoprecipitated using a
Techniques: Expressing, Mutagenesis